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1Department of Bioengineering, School of Engineering and Applied Science,University of Pennsylvania, 2Center for Brain Injury & Repair, Department of Neurosurgery, Perelman School of Medicine,University of Pennsylvania, 3Center for Neurotrauma, Neurodegeneration & Restoration,Michael J. Crescenz Veterans Affairs Medical Center, 4School of Biomedical Engineering,Drexel University
Chapters
- 00:05Title
- 00:58Micro-column Fabrication Using Capillary Tubes
- 02:04Micro-column Trimming and Sterilization
- 02:48Preparation of the Pyramidal Micro-well Array
- 04:46Formation of Neuronal Aggretates
- 05:27ECM Core Fabrication
- 06:42Neuronal Aggregate Seeding of Micro-columns
- 08:14Results: Observation of Axonal Growth in Micro-TENNs by Phase-contrast Imaging and Cytoarchitecture by Confocal Microscopy
- 09:51Conclusion
Dette manuskript beskriver fremstillingen af mikrovæv-manipulerede neurale netværk: tredimensionale mikronstørrelseskonstruktioner bestående af langjusterede aksonale kanaler, der spænder over aggregerede neuronal populationer indkapslet i en rørformet hydrogel. Disse levende stilladser kan fungere som funktionelle relæer til rekonstruktion eller modulering af neurale kredsløb eller som biofideliske test-senge, der efterligner grå-hvid stof neuroanatomi.
