/

/

的多孔冷冻管省去冻结工件时肌肉组织在液氮中直接浸入

JoVE 신문
생물학

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

JoVE 신문 생물학

A Multi-hole Cryovial Eliminates Freezing Artifacts when Muscle Tissues are Directly Immersed in Liquid Nitrogen

Please note that all translations are automatically generated. Click here for the English version.

的多孔冷冻管省去冻结工件时肌肉组织在液氮中直接浸入

DOI:

10.3791/55616-v

•

06:42 min

•

April 06, 2017

•

Yizhong Huang, Maozhang He, Qingjie Zeng, Lin Li, Zhen Zhang, Junwu Ma, Yanyu Duan

¹State Key Laboratory for Pig Genetic Improvement and Production Technology,Jiangxi Agricultural University

Chapters

00:05Title
01:17Equipment for Sample Collection and Preparation of Muscle Samples
02:47Freezing and Storing Muscles, and Slide Preparation
04:40Results: Freezing Muscle Tissue Using a Multi-hole Cryovial
06:10Conclusion

Summary

자동 번역

这个协议描述的过程通过直接让他们与液氮冷冻的肌肉组织。该协议还突出一个新的离心管，可避免氮气的“毯效应”，当液氮接触样本的组织表面上。

Tags

AI-assisted Freezing Cryogenic Sample Preparation Multi-hole Cryovial Liquid Nitrogen Freezing Freezing Artifact Elimination Cryostat Sectioning

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

冻结人类胚胎干细胞

人类胚胎干细胞冷冻和解冻

人鼻粘膜上皮细胞在气液界面培养

Aip1p动力由R256H突变的肌动蛋白蚀变

组织分流和冻结的骨骼肌疾病模型

串联高压冷冻和植物组织中快速冷冻替代透射电子显微镜

暴跌冻结：在透射电子显微镜的超微结构和免疫组化研究工具悬架细胞

吸收荧光、磁性纳米微粒测定昆虫的吸液能力

使用夹层冷冻装置快速冷冻，在电子显微镜下对生物标本进行良好的超微结构保存

Directly Measuring Forces Within Reconstituted Active Microtubule Bundles

Read Article