Journal
/
의학
/
Laparoscopic Technique for Serial Collection of Liver and Mesenteric Lymph Nodes in Macaques
JoVE 신문
의학
This content is Free Access.
JoVE 신문 의학
Laparoscopic Technique for Serial Collection of Liver and Mesenteric Lymph Nodes in Macaques

Laparoscopic Technique for Serial Collection of Liver and Mesenteric Lymph Nodes in Macaques

DOI:
10.3791/55617-v

•

11:06 min

•

May 02, 2017

•

, , , , , , , , , ,
1Department of Pharmaceutics,Washington National Primate Research Center, University of Washington, 2Division of Primate Resources,Washington National Primate Research Center, University of Washington

Chapters

  • 00:05Title
  • 01:12Pre-surgical Preparation
  • 01:49Laparoscopic Entry and Cannula Placement
  • 04:17Mesenteric Lymph Node Biopsy
  • 06:38Liver Biopsy
  • 07:53Results: Identification of Leukocyte and Lymphocyte Populations in Mesenteric Lymph Node and Liver Biopsies
  • 09:24Conclusion

Summary

자동 번역

자동 번역

Here, we describe a minimally invasive laparoscopic technique for serial sampling of liver and mesenteric lymph nodes (MLN) in macaques that allows for increased sampling frequency, and reduces the potential for surgical complications when compared to performing a laparotomy.

Tags

Laparoscopic TechniqueSerial CollectionLiverMesenteric Lymph NodesMacaquesMinimally InvasiveGI TractMicrobial TranslocationHIV BiologyImmunological ConsequencesPathologyMultiple Time PointsAnimal WelfareSterile ProcedureScalpel IncisionVarus NeedlePneumoperitoneumCO2 Insufflator

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

Technique of Subnormothermic Ex Vivo Liver Perfusion for the Storage, Assessment, and Repair of Marginal Liver Grafts

Collection and Extraction of Saliva DNA for Next Generation Sequencing

High-frequency Ultrasound Imaging of Mouse Cervical Lymph Nodes

Intraoperative Gastroscopy for Tumor Localization in Laparoscopic Surgery for Gastric Adenocarcinoma

Long-Term Catheterization of the Intestinal Lymph Trunk and Collection of Lymph in Neonatal Pigs

Laparoscopic Radical Left Pancreatectomy for Pancreatic Cancer: Surgical Strategy and Technique Video

Murine Precision-Cut Liver Slices as an Ex Vivo Model of Liver Biology

Flow Cytometry Analysis of Immune Cell Subsets within the Murine Spleen, Bone Marrow, Lymph Nodes and Synovial Tissue in an Osteoarthritis Model

Laparoscopic Pancreatoduodenectomy for Pancreatic Cancer Using In-Situ No-Touch Isolation Technique

Fluorescent Laparoscopic Central Hepatectomy for Liver Cancer Using Indocyanine Green Negative Staining

Read Article