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利用DNA染色体引发的侧群体表型，从生物样品中检测和纯化癌细胞

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Harnessing the DNA Dye-triggered Side Population Phenotype to Detect and Purify Cancer Stem Cells from Biological Samples

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利用DNA染色体引发的侧群体表型，从生物样品中检测和纯化癌细胞

DOI:

10.3791/55634-v

•

09:57 min

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May 10, 2017

•

Maximilian Boesch*^1,2,3, Elisabeth Hoflehner³, Dominik Wolf^3,4, Guenther Gastl, Sieghart Sopper*^2,3

¹Institute of Immunobiology,Kantonsspital St. Gallen, ²Internal Medicine V,Medical University of Innsbruck, ³Tyrolean Cancer Research Institute (TKFI), ⁴Medical Clinic III, Oncology, Hematology, Immunoncology and Rheumatology,University Clinic Bonn (UKB)

Chapters

00:05Title
01:41Cell Preparation
02:51Sample Preparation and Staining
05:44Flow Cytometry Analysis
07:24Results: Identification and Characterization of Side Population Cells
08:20Conclusion

Summary

자동 번역

允许从生物样品中鉴定和分离干细胞群体的方法对于癌症及其以外的干细胞靶向治疗的进展至关重要。在这里，我们提供使用染料触发侧群体表型的癌症干细胞分离的详细方案。

Tags

DNA Dye Side Population Cancer Stem Cells ABC Drug Transporters Cell Culture Cell Suspension Trypsin-EDTA Hemocytometer Violet Dye Inhibition Controls

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