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将细胞质Foci分类为哺乳动物应激颗粒的方法
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Methods to Classify Cytoplasmic Foci as Mammalian Stress Granules
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将细胞质Foci分类为哺乳动物应激颗粒的方法

DOI:
10.3791/55656-v

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09:33 min

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May 12, 2017

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, , , , ,
1Division of Rheumatology, Immunology, and Allergy,Brigham and Women’s Hospital, 2Department of Medicine,Harvard Medical School, 3Department of Histology and Embryology,Poznan University of Medical Sciences, 4The Broad Institute of Harvard and M.I.T.

Chapters

  • 00:05Title
  • 00:52Stressing Cells
  • 02:19Cell Fixation and Staining
  • 05:23Fluorescence In Situ Hybridization
  • 07:43Results: Stress Granules Formation in Sodium Arsenite- and Vinorelbine-treated Cells
  • 08:46Conclusion

Summary

자동 번역

자동 번역

应激颗粒（SG）是在暴露于各种应激的细胞中形成的非膜细胞质结构。 SGs包含mRNA，RNA结合蛋白，小核糖体亚基，翻译相关因子和各种细胞信号传导蛋白。该协议描述了一种使用几种实验方法来检测，表征和量化真正的 SG的工作流程。

Tags

Cytoplasmic FociStress GranulesMammalian CellsU-2 OS CellsSodium ArseniteVinorelbineCycloheximidePuromycinImmunofluorescenceCell FixationCell Permeabilization

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