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Brain Membrane Fractionation: An Ex Vivo Approach to Assess Subsynaptic Protein Localization
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Brain Membrane Fractionation: An Ex Vivo Approach to Assess Subsynaptic Protein Localization

Brain Membrane Fractionation: An Ex Vivo Approach to Assess Subsynaptic Protein Localization

DOI:
10.3791/55661-v

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09:49 min

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May 12, 2017

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, , , ,
1Unitat de Farmacologia, Departament Patologia i Terapèutica Experimental, Facultat de Medicina, IDIBELL,Universitat de Barcelona, L’Hospitalet de Llobregat, 2Institut de Neurociències,Universitat de Barcelona, 3Center for Neurosciences of Coimbra, Institute of Biochemistry, Faculty of Medicine,University of Coimbra

Chapters

  • 00:00Title
  • 00:18Introduction
  • 02:22Obtaining Mouse Brain Synaptosomes Using a Discontinuous Sucrose Gradient
  • 04:37Pre-post and Extra-synaptic Fraction Isolation
  • 06:52Analyze Samples and Validate Fractionation
  • 07:26Representative Results
  • 08:53Conclusion

Summary

자동 번역

자동 번역

Here, we present a brain membrane fractionation protocol that represents a robust procedure to isolate proteins belonging to different synaptic compartments.

Tags

Brain Membrane FractionationSynaptic CompartmentsSynaptic TransmissionElectron MicroscopyHippocampusHomogenizationSucrose Calcium SolutionCentrifugationSynaptosomeSubsynaptic CompartmentsDetergent SolubilizationPHImmunoblot Analysis

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