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Chapters
- 00:05Title
- 01:19Electronic Cigarette-vapor Chamber Assembly
- 02:56Tobacco Cigarette-smoke Chamber Assembly
- 04:25Animal Handling
- 05:05Operating the E-cigarette Chamber
- 06:50Operating the Tobacco Cigarette Chamber
- 08:41Results: Clinically-relevant Measurement of CO and Cotinine Resulting from E-vapor and Cigarette Smoke Exposure
- 09:39Conclusion
This protocol describes a method for exposing rodents to electronic cigarette vapor (E-vapor) and cigarette smoke. Exposure chambers are constructed by modifying anesthesia chambers with an automated pumping system that delivers E-vapor or cigarette smoke to rodents. This system can easily be modified to accommodate many experimental endpoints.
