/

/

分离的原发性上皮细胞从附睾的全细胞贴片钳记录

JoVE 신문
발생학

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

JoVE 신문 발생학

Whole-cell Patch-clamp Recordings of Isolated Primary Epithelial Cells from the Epididymis

Please note that all translations are automatically generated. Click here for the English version.

分离的原发性上皮细胞从附睾的全细胞贴片钳记录

DOI:

10.3791/55700-v

•

11:52 min

•

August 03, 2017

•

Bao Li Zhang^2,3, Da Yuan Gao^2,3, Xiao Xu Zhang³, Shuo Shi, Winnie Shum

¹School of Life Science and Technology,ShanghaiTech University, ²Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences, ³University of Chinese Academy of Sciences, ⁴Shanghai Institute for Advanced Immunochemical Studies,ShanghaiTech University

Chapters

00:05Title
00:59Preparation of Dissection Instruments and Reagents
02:10Isolation of Epithelial Cells from Rat Cauda Epididymides
06:09Patch-clamp Experiment
08:32Results: Typical Whole-cell Curent in Single Epididymal Principal Cells
10:03Conclusion

Summary

자동 번역

我们提出了一种协议，结合细胞分离和全细胞膜片钳记录来测量主要解离的上皮细胞从大鼠cauda附睾的电性质。该方案允许研究原发性附睾上皮细胞的功能特性，以进一步阐明附睾的生理作用。

Tags

Whole-cell Patch-clamp Primary Epithelial Cells Epididymis Reproductive Biology Sperm Maturation Paternal Traits Electrical Properties Collagenase Cell Dissociation

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

使用分离线粒体从小鼠骨骼肌进行高通量微孔板呼吸测量最小数量

日本鹌鹑胚胎的卵黄囊膜主内胚上皮细胞培养

从培养儿童原发性鼻腔上皮细胞和重新编程为诱导多能干细胞

的发展<em>体外</em>检测以确定间充质细胞将经历上皮 - 间质转化的收缩功能

体外测定菌落为表征的三效祖细胞从成人胰腺小鼠隔离

诱导多能干细胞使用成纤维样滑膜生成类风湿关节炎患者的关节分离

在肉瘤细胞间质上皮转变的诱导

人胚胎或诱导多能干细胞对视网膜色素上皮细胞的快速定向分化

人成体和胎心标本中原发性心外膜细胞的分离培养

秀丽线虫生殖的计算分析研究细胞核、蛋白质和细胞骨架的分布

Read Article