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1School of Life Science and Technology,ShanghaiTech University, 2Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences, 3University of Chinese Academy of Sciences, 4Shanghai Institute for Advanced Immunochemical Studies,ShanghaiTech University
Chapters
- 00:05Title
- 00:59Preparation of Dissection Instruments and Reagents
- 02:10Isolation of Epithelial Cells from Rat Cauda Epididymides
- 06:09Patch-clamp Experiment
- 08:32Results: Typical Whole-cell Curent in Single Epididymal Principal Cells
- 10:03Conclusion
Wir präsentieren ein Protokoll, das Zellisolation und Ganzzell-Patch-Clamp-Aufzeichnung kombiniert, um die elektrischen Eigenschaften der primär dissoziierten Epithelzellen aus den Ratten-Cauda-Epididymiden zu messen. Dieses Protokoll erlaubt die Untersuchung der funktionellen Eigenschaften von primären epididymalen Epithelzellen, um die physiologische Rolle des Nebenhodens weiter zu erforschen.
