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Murine Short Axis Ventricular Heart Slices for Electrophysiological Studies
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JoVE 신문 의학
Murine Short Axis Ventricular Heart Slices for Electrophysiological Studies
DOI:

07:51 min

June 04, 2017

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Chapters

  • 00:05Title
  • 00:55Mouse Heart Isolation and Ventricle Embedding
  • 02:30Slicing the Ventricle Tissue
  • 03:47Sharp Electrode Setup Preparation and Action Potential Recordings
  • 05:51Results: Electrical Integration of Transplanted Induced Pluripotent Stem Cell iPSCM
  • 06:45Conclusion

Summary

자동 번역

Here, we describe the preparation of viable ventricular slices from adult mice and their use for sharp electrode action potential recordings. These multicellular preparations provide a preserved in vivo like tissue structure, which makes them a valuable model for electrophysiological and pharmacological studies in vitro.

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