Glucose Uptake Measurement and Response to Insulin Stimulation in <em>In Vitro</em> Cultured Human Primary Myotubes

00:05Title
00:34Cell Culture Media and Solution Preparation
02:45Culture of Human Primary Muscle Cells
04:19Insulin Stimulation and Glucose Uptake
05:30Cell Lysis
06:06Determination of Radiolabeled Glucose
06:52Results: Effective Glucose Uptake Measurement and Analysis of Response to Insulin Stimulation
07:37Conclusion

Summary

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In this method, human primary muscle cells are cultured in vitro to obtain differentiated myotubes and glucose uptake rates are measured. We provide a detailed protocol to quantify rates in basal and insulin-stimulated states using radiolabeled [³H] 2-deoxy-D-Glucose.

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Glucose Uptake Measurement and Response to Insulin Stimulation in In Vitro Cultured Human Primary Myotubes

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