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1CarMeN Laboratory, INSERM U1060, INRA 1397,University of Lyon, 2Department of digestive and bariatric surgery, Obesity Integrated Center, University Hospital of Edouard Herriot, Hospices Civils de Lyon,Lyon 1 University, 3Division of Endocrinology, Diabetes, Hypertension and Nutrition, Department of Clinical Medicine, Faculty of Medicine,University of Geneva
Chapters
- 00:05Title
- 00:34Cell Culture Media and Solution Preparation
- 02:45Culture of Human Primary Muscle Cells
- 04:19Insulin Stimulation and Glucose Uptake
- 05:30Cell Lysis
- 06:06Determination of Radiolabeled Glucose
- 06:52Results: Effective Glucose Uptake Measurement and Analysis of Response to Insulin Stimulation
- 07:37Conclusion
Bij deze werkwijze worden humane primaire spiercellen in vitro gekweekt om gedifferentieerde myotubes te verkrijgen en de opname van glucose wordt gemeten. Wij bieden een gedetailleerd protocol om de tarieven in basale en insuline gestimuleerde staten te kwantificeren met radioactief gemerkte [3H] 2-deoxy-D-glucose.
