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Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve
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Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve

Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve

DOI:
10.3791/55762-v

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09:13 min

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June 14, 2017

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, , , , , ,
1Centro Cardiologico Monzino IRCCS, 2Cardiovascular Tissue Bank of Milan,Centro Cardiologico Monzino IRCCS, 3Department of Clinical Sciences and Community Health, Cardiovascular Section,University of Milan, 4Department of Cardiovascular Disease, Development and Innovation Cardiac Surgery Unit,Centro Cardiologico Monzino IRCCS

Chapters

  • 00:05Title
  • 01:17Mitral Valve Preparation
  • 03:39Protein Extraction
  • 07:20Results: Four Newly Identified Mitral Valve Proteins
  • 08:28Conclusion

Summary

자동 번역

자동 번역

The protein composition of the human mitral valve is still partially unknown, because its analysis is complicated by low cellularity and therefore by low protein biosynthesis. This work provides a protocol to efficiently extract protein for the analysis of the mitral valve proteome.

Tags

Keywords: Protein ExtractionMitral ValveCardiac Valve DiseaseProteomic AnalysisDiagnostic MarkersTherapeutic TargetsPorcine Mitral ValveExplanted HeartLeft AtriumLeft VentricleAnterior Mitral Valve LeafletPosterior Mitral Valve LeafletCommissuresDissectionProtein Extraction Workflow

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