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Bioengineering

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JoVE Journal Bioengineering
Et integreret System til at fjernt udløse intracellulære signaltransduktion af Upconversion nanopartikel-medieret Kinase Photoactivation
 
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Et integreret System til at fjernt udløse intracellulære signaltransduktion af Upconversion nanopartikel-medieret Kinase Photoactivation

Article DOI: 10.3791/55769-v • 11:20 min • August 30th, 2017
August 30th, 2017 •
1,2, 1, 1,2, 3, 1,2, 1
1Institute of Chemistry, Academia Sinica, 2Department of Chemistry, National Taiwan University, 3Department of Materials and Mineral Resources Engineering, National Taipei University of Technology

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I denne protokol, bur protein kinase A (PKA), en cellulær signaltransduktion bioeffector, var immobiliseret på en nanopartikel overflade, microinjected ind i cytosol, og aktiveres af upconverted UV-lys fra nær-infrarødt (NIR) bestråling, inducerende downstream stress fiber opløsning i cytosol.

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Bioteknologi sag 126 Caged enzym cellulære reaktion mikroinjektion fotolyse protein kinase A upconversion nanopartikler photoactivation
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