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Chapters
- 00:00Title
- 00:57Solid-state NMR sample production
- 04:02Preliminary structural characterization based on 1D solid-state NMR
- 07:36Conformational analysis and 3D structural determination
- 13:01Results
- 14:15Conclusion
Structures of supramolecular protein assemblies at atomic resolution are of high relevance because of their crucial roles in a variety of biological phenomena. Herein, we present a protocol to perform high-resolution structural studies on insoluble and non-crystalline macromolecular protein assemblies by magic-angle spinning solid-state nuclear magnetic resonance spectroscopy (MAS SSNMR).
