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1Curriculum in Toxicology,University of North Carolina at Chapel Hill, 2National Toxicology Program Laboratory (NTPL), DNTP,National Institute of Environmental Health Sciences, 3Cellular and Molecular Pathology Branch, DNTP,National Institute of Environmental Health Sciences, 4Charles River Laboratories Inc.
Chapters
- 00:05Title
- 01:08Removal of Mammary Whole Mount from a Glass Slide
- 02:30Tissue Processing
- 03:52Embedding the Processed Mammary Tissue
- 05:10Sectioning the Paraffin-embedded Mammary Tissue on the Microtome and Automated H and E Staining
- 06:51Results: Lesion Identification in Whole Mount Mammary Gland Sections
- 08:23Conclusion
We developed a method to successfully remove, process, section, and stain, for histopathological evaluation, mammary tissue that had originally been fixed on slides as whole mounts. This method may promote the collection and evaluation of mammary gland whole mounts in reproductive and developmental test guideline studies.
