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从白蛉提取内分泌物的方法<em> Bemisia tabaci</em

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Methods for the Extraction of Endosymbionts from the Whitefly Bemisia tabaci

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从白蛉提取内分泌物的方法<em> Bemisia tabaci</em

DOI:

10.3791/55809-v

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07:28 min

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June 19, 2017

•

Dan-Tong Zhu, Xin-Ru Wang, Fei-Xue Ban, Chi Zou, Shu-Sheng Liu, Xiao-Wei Wang

¹Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogen and Insects, Institute of Insect Sciences,Zhejiang University

Chapters

00:05Title
00:52Whitefly Rearing, Cryptic Species Identification, and Endosymbiont Identification and Localization
02:11Whitefly Bacteriome Dissection and Purification
03:47Amplification of Endosymbiont Genomes
05:02Results: Extraction and Genome Sequencing of Endosymbionts from the Whitefly Bemisia tabaci
06:12Conclusion

Summary

자동 번역

在这里，我们提出了通过解剖和过滤从粉虱烟粉虱中分离内分泌物的方案。扩增后，DNA样品适用于后续测序和研究内共生与粉虱之间的共生关系。

Tags

Keywords: Endosymbionts Whitefly Bemisia Tabaci PCR DNA Extraction Bacteriome Dissection Insect Endosymbionts Bacterial Isolation

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