Journal
/
생화학
/
In Vitro Polymerization of F-actin on Early Endosomes
JoVE 신문
생화학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생화학
In Vitro Polymerization of F-actin on Early Endosomes

In Vitro Polymerization of F-actin on Early Endosomes

DOI:
10.3791/55829-v

•

12:15 min

•

August 28, 2017

•

, , , ,
1Department of Biochemistry,University of Geneva, 2Department of Fundamental Microbiology,University of Lausanne

Chapters

  • 00:05Title
  • 00:53Endosomal Fraction Preparation
  • 06:10In Tube Assay for Endosome-dependent Actin Polymerization
  • 08:11In Chamber Assay for Endosome-dependent Actin Polymerization
  • 09:29Results: F-actin Polymerization on Early Endosomes
  • 11:09Conclusion

Summary

자동 번역

자동 번역

Early endosome functions depend on F-actin polymerization. Here, we describe a microscopy-based in vitro assay that reconstitutes the nucleation and polymerization of F-actin on early endosomal membranes in test tubes, thus rendering this complex series of reactions amenable to biochemical and genetic manipulations.

Tags

Keywords: In VitroF-actinPolymerizationEarly EndosomesMembrane TraffickingActin FieldsAcidic PathwayNucleationReconstitutionHeLa CellsHomogenization BufferCell FractionationPhase Contrast Microscopy

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/55829?article_type=v

Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics
check_url/kr/55829?article_type=v

Measuring Protein Binding to F-actin by Co-sedimentation
check_url/kr/55829?article_type=v

A Simple Method for High Throughput Chemical Screening in Caenorhabditis Elegans
check_url/kr/55829?article_type=v

Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-1
check_url/kr/55829?article_type=v

Assessing the Viability of a Synthetic Bacterial Consortium on the In Vitro Gut Host-microbe Interface
check_url/kr/55829?article_type=v

An In Vitro Assay to Detect tRNA-Isopentenyl Transferase Activity
check_url/kr/55829?article_type=v

In Vitro Reconstitution of Self-Organizing Protein Patterns on Supported Lipid Bilayers
check_url/kr/55829?article_type=v

Quantification of Hypopigmentation Activity In Vitro
check_url/kr/55829?article_type=v

In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones
check_url/kr/55829?article_type=v

Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy

Read Article