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一种优化的血凝抑制 (HI) 测定流感特异抗体滴

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An Optimized Hemagglutination Inhibition (HI) Assay to Quantify Influenza-specific Antibody Titers

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一种优化的血凝抑制 (HI) 测定流感特异抗体滴

DOI:

10.3791/55833-v

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06:34 min

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December 01, 2017

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Lukas Kaufmann*¹, Mohammedyaseen Syedbasha*¹, Dominik Vogt, Yvonne Hollenstein, Julia Hartmann, Janina E. Linnik^2,3, Adrian Egli⁴

¹Applied Microbiology Research, Department of Biomedicine,University of Basel, ²Department of Biosystems Science and Engineering,ETH Zurich, ³Swiss Institute of Bioinformatics, ⁴Clinical Microbiology,University Hospital Basel

Chapters

00:05Title
00:54Hemagglutination Inhibition (HI) Assay
03:28Results: Representative Vacination-induced Antibody Responses Against Influenza
05:11Conclusion

Summary

자동 번역

本协议描述了如何进行血凝抑制试验, 以量化流感疫苗接受者血清样本中的流感特异性抗体滴。第一种方法是通过血凝法确定最佳病毒抗原浓度。第二种方法通过血凝抑制来量化流感特异抗体滴。

Tags

Keywords: Hemagglutination Inhibition Assay Influenza-specific Antibody Titers Vaccine-mediated Immunity Neutralizing Antibodies Mouse Serum Culture Supernatants Antigen Solution RDE Treated Serum Samples Antiserum Serial Dilutions Red Blood Cell Solution Incubation Time Hemagglutination

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