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人死后脑中洗涤剂不溶性蛋白的富集

JoVE 신문
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Enrichment of Detergent-insoluble Protein Aggregates from Human Postmortem Brain

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人死后脑中洗涤剂不溶性蛋白的富集

DOI:

10.3791/55835-v

•

09:35 min

•

October 24, 2017

•

Ian Diner, Tram Nguyen, Nicholas T. Seyfried

¹Department of Biochemistry,Emory School of Medicine

Chapters

00:05Title
00:42Homogenization and Fractionation
04:54Western Blotting
07:48Results: Detection of AD-related Detergent-insoluble Protein Aggregates from the Human Postmortem Brain
09:05Conclusion

Summary

자동 번역

本文介绍了一种从人死后大脑中浓缩洗涤剂不溶性蛋白集料的简化分馏方法。

Tags

Keywords: Detergent-insoluble Protein Aggregates Human Postmortem Brain Neurodegeneration Misfolded Protein Aggregates Gray Matter Low-salt Buffer Sodium Chloride Sarkosyl Sonication Protein Concentration

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