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Genoom-wide mapping van eiwit-DNA-interacties met ChEC-seq in<em> Saccharomyces cerevisiae</em
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JoVE 신문 유전학
Genome-wide Mapping of Protein-DNA Interactions with ChEC-seq in Saccharomyces cerevisiae
DOI:

10:43 min

June 03, 2017

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Chapters

  • 00:05Title
  • 00:49Chromatin Endogenous Cleavage (ChEC)
  • 06:30Size Selection
  • 08:28Results: ChEC-seq Enables Genome-wide Mapping of Protein-DNA Interactions
  • 09:54Conclusion

Summary

자동 번역

We beschrijven chromatin endogene splitsing gekoppeld aan high-throughput sequencing (ChEC-seq), een chromatine immunoprecipitatie (ChIP) -orthogonale methode voor het mappen van eiwitbindingsplaatsen genoom-wijd met micrococculaire nuclease (MNase) fusie-eiwitten.

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