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Tuning in the Hippocampal Theta Band In Vitro: Methodologies for Recording from the Isolated Rodent Septohippocampal Circuit
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Tuning in the Hippocampal Theta Band In Vitro: Methodologies for Recording from the Isolated Rodent Septohippocampal Circuit

Tuning in the Hippocampal Theta Band In Vitro: Methodologies for Recording from the Isolated Rodent Septohippocampal Circuit

DOI:
10.3791/55851-v

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11:37 min

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August 02, 2017

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,
1Department of Psychiatry, Douglas Mental Health University Institute,McGill University

Chapters

  • 00:05Title
  • 00:54Isolating the Hippocampus
  • 03:47Set Up Fast Perfusion for Recording the Isolated Hippocampus
  • 04:34Extracellular Recording on In Vitro Hippocampal Theta Oscillations
  • 07:57Optogenetic Control in the Isolated Hippocampus
  • 09:11Results: Cell-type Specific Activity of Pyramidal Cell and PV Interneuron During Spontaneous Theta Oscillations in the Intact Hippocampal Preparation from a PV-TOM Mouse
  • 10:19Conclusion

Summary

자동 번역

자동 번역

Here, we present a protocol for recording rhythmic neuronal network theta and gamma oscillations from an isolated whole hippocampal preparation. We describe the experimental steps from extraction of the hippocampus to details of field, unitary and whole-cell patch clamp recordings as well as optogenetic pacing of the theta rhythm.

Tags

HippocampusTheta BandIn VitroSeptohippocampal CircuitField RecordingsUnitary RecordingsPatch-clamp RecordingsOptogenetic StimulationRhythmic Neuronal NetworkLearning And MemoryOscillations

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