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来自新世界Zika病毒感染性克隆的重组病毒的救援和表征

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Rescue and Characterization of Recombinant Virus from a New World Zika Virus Infectious Clone

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来自新世界Zika病毒感染性克隆的重组病毒的救援和表征

DOI:

10.3791/55857-v

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07:35 min

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June 07, 2017

•

James Weger-Lucarelli, Nisha K. Duggal, Aaron C. Brault, Brian J. Geiss, Gregory D. Ebel

¹Department of Microbiology, Immunology, and Pathology,Colorado State University, ²Division of Vector-Borne Diseases,Centers for Disease Control and Prevention

Chapters

00:05Title
00:40Preparation of In Vitro Transcribed RNA
02:54Rescue of Infectious Virus by Electroporation
05:56Results: In Vitro and In Vivo Characteristics of Clone-derived Virus
07:02Conclusion

Summary

자동 번역

该方案描述了从双质粒感染性cDNA克隆中恢复感染性Zika病毒。

Tags

Keywords: Zika Virus Infectious Clone Recombinant Virus Flavivirus Plasmid Digestion Ligation In Vitro Transcription Vero Cells Electroporation

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