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生物活性蛋白或多肽在水凝胶上的应用

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Patterning Bioactive Proteins or Peptides on Hydrogel Using Photochemistry for Biological Applications

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生物活性蛋白或多肽在水凝胶上的应用

DOI:

10.3791/55873-v

•

09:19 min

•

September 15, 2017

•

Taylor B. Dorsey^2,3, Alexander Grath², Cancan Xu, Yi Hong, Guohao Dai^2,3

¹Department of Biomedical Engineering,Rensselaer Polytechnic Institute, ²Center for Biotechnology and Interdisciplinary Studies,Rensselaer Polytechnic Institute, ³Department of Bioengineering,Northeastern University, ⁴Department of Bioengineering,University of Texas at Arlington

Chapters

00:05Title
00:52Preparation of Materials for Hydrogel Synthesis
01:57Preparation of Sterile Glass Slide Molds for Hydrogel Formation
03:04Hydrogel Formation
04:06Protein Patterning
05:18Cell Seeding onto Hydrogels
06:58Results: Endothelial Cells Respond to VEGF Patterned Hydrogels
08:31Conclusion

Summary

자동 번역

在这个方法中, 我们使用聚合和点击化学技术在聚乙二醇 (PEG) 水凝胶表面创造蛋白质或肽模式, 提供固定化的生物活性信号来研究细胞反应体外.

Tags

Keywords: Hydrogel Bioactive Proteins Peptides Photochemistry Spatial Protein Patterning Regenerative Medicine Cell Signaling Stem Cell Fate Polyethylene Glycol Diacrylate Photoinitator Fibronectin Hydrogel Mold UV Sterilization

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