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ODELAY:用于酵母生长多参数定量的大规模方法
JoVE 신문
생물학
This content is Free Access.
JoVE 신문 생물학
ODELAY: A Large-scale Method for Multi-parameter Quantification of Yeast Growth
DOI:

11:19 min

July 03, 2017

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Chapters

  • 00:05Title
  • 00:39Agar Mold Assembly
  • 03:36ODELAY Culture Preparation
  • 05:41Spotting on Agar Using Automated Liquid Spotting Robot
  • 07:21ODELAY Time-lapse Microscopy
  • 09:03Results: ODELAY Characterizes all 3 Growth Phase of Yeast Cells on Solid Media
  • 10:00Conclusion

Summary

자동 번역

我们提出了一种定量单个酵母细胞的生长表型的方法,它们使用称为酵母生活阵列的单细胞倍增评估(ODELAY)的延时显微镜在固体培养基上生长成菌落。可以直接观察和量化生长到菌落中的遗传相同细胞的群体异质性。

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