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Genetics

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JoVE Journal Genetics
选择依赖和独立生成CRISPR / Cas9介导的哺乳动物细胞基因敲除
 
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选择依赖和独立生成CRISPR / Cas9介导的哺乳动物细胞基因敲除

Article DOI: 10.3791/55903-v • 11:35 min • June 16th, 2017
June 16th, 2017 •
1, 1, 1
1Cell Biology and Neuroscience, University of California, Riverside

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遗传操纵体细胞系的能力的最新进展对于基础和应用研究具有巨大的潜力。在这里，我们提出了CRISPR / Cas9在哺乳动物细胞系中产生敲除生产和筛选的两种方法，使用和不使用选择标记。

Tags

遗传学，第124期，CRISPR / Cas9，同源驱动修复，非同源末端连接，斑点印迹，克隆选择，筛选
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