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1Department of Medicine, Institute of Translational Pharmacology,Italian National Research Council, 2Epigenetics and Regenerative Medicine,IRCCS Fondazione Santa Lucia, 3Biological and Environmental Sciences and Engineering Division,King Abdullah University of Science and Technology (KAUST), 4Department of Life Sciences,University of Modena and Reggio Emilia
Chapters
- 00:05Title
- 01:00Muscle Injury and Tissue Harvest
- 03:12Cryosectioning, Immunostaining, and Confocal Microscopy Imaging
- 06:47Results: Representative Injured Muscle Tissue and Muscle Satellite Cell (MuSC) Analyses
- 07:43Conclusion
Aktive Autophagie ist mit produktiven Muskelregeneration verbunden, die für die Muskelstammzell-Aktivierung (MuSC) essentiell ist. Hier stellen wir ein Protokoll für die in situ- Detektion von LC3, einen Autophagie-Marker in MyoD-positiven MuSCs von Muskelgewebeabschnitten von Kontroll- und verletzten Mäusen zur Verfügung.
