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Journal / Developmental Biology / 通过机械组织分离和流式细胞术对哺乳动物雄性生殖细胞多特异性纯化的标准化方法…
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Developmental Biology

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JoVE Journal Developmental Biology
通过机械组织分离和流式细胞术对哺乳动物雄性生殖细胞多特异性纯化的标准化方法
 
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通过机械组织分离和流式细胞术对哺乳动物雄性生殖细胞多特异性纯化的标准化方法

Article DOI: 10.3791/55913-v • 08:46 min • July 12th, 2017
July 12th, 2017 •
*1,2,3,4, *1, 1, 1, 3,4, 1
1Department of Genetics, Washington University School of Medicine, 2Graduate Program in Areas of Basic and Applied Biology (GABBA), Abel Salazar Institute of Biomedical Sciences, University of Porto, 3Instituto de Investigação e Inovação em Saúde, University of Porto, 4IPATIMUP - Instituto de Patologia e Imunologia Molecular da Universidade do Porto, 5Department of Pathology & Immunology, Washington University School of Medicine
* These authors contributed equally

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这项工作描述了从不同哺乳动物物种的睾丸组织获得纯化的生殖细胞群体的方法的标准化。结合机械睾丸解离，染色与Hoechst-33342和碘化丙啶和FACS分选是一个简单的方案，广泛应用于男性生殖生物学的比较研究。

Tags

发育生物学，第125期，比较繁殖，荧光活化细胞分选（FACS），Hoechst-33342，雄性生殖细胞，机械解离，精子发生，睾丸，
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