Journal
/
발생학
/
Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement
JoVE 신문
발생학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 발생학
Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement

Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement

DOI:
10.3791/55918-v

•

10:45 min

•

July 26, 2017

•

, , ,
1Department of Orthopedic Surgery,Geneva University Hospitals & Faculty of Medicine, 2Department of Basic Neurosciences,Geneva Medical Center, 3Department of Cell Physiology and Metabolism,Geneva Medical Center

Chapters

  • 00:05Title
  • 01:07Dissociation Protocol
  • 02:42Antibody Staining and Cell Sorting
  • 04:39Transfection
  • 05:57Ca2+ Imaging in Differentiated Cells and Image Analysis
  • 08:50Results: Myoblast Characterization and a SOCE Experiment
  • 09:54Conclusion

Summary

자동 번역

자동 번역

Here, we describe a methodology to obtain a pure population of human myoblasts from adult muscle tissue. These cells are used to study in vitro skeletal muscle differentiation and, in particular, to study proteins involved in Ca2+ signaling.

Tags

Keywords: Human MyoblastsMyogenic DifferentiationStore-operated Calcium EntrySkeletal MuscleMuscle RegenerationIonic ChannelsTranscription FactorsMuscular DystrophiesTrypsinCell CultureCell IsolationCell Staining

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/55918?article_type=v

Isolation and Quantitative Immunocytochemical Characterization of Primary Myogenic Cells and Fibroblasts from Human Skeletal Muscle
check_url/kr/55918?article_type=v

Analysis of Zebrafish Larvae Skeletal Muscle Integrity with Evans Blue Dye
check_url/kr/55918?article_type=v

Differentiation of the SH-SY5Y Human Neuroblastoma Cell Line
check_url/kr/55918?article_type=v

Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues
check_url/kr/55918?article_type=v

Preparation and Culture of Myogenic Precursor Cells/Primary Myoblasts from Skeletal Muscle of Adult and Aged Humans
check_url/kr/55918?article_type=v

Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells
check_url/kr/55918?article_type=v

Isolation, Culture, and Differentiation of Bone Marrow Stromal Cells and Osteoclast Progenitors from Mice
check_url/kr/55918?article_type=v

Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells
check_url/kr/55918?article_type=v

Isolation, Propagation, and Prion Protein Expression During Neuronal Differentiation of Human Dental Pulp Stem Cells
check_url/kr/55918?article_type=v

Isolation, Culture, Characterization, and Differentiation of Human Muscle Progenitor Cells from the Skeletal Muscle Biopsy Procedure

Read Article