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高分子量生物素化葡聚糖胺在大鼠丘脑-皮质投影示踪中的应用

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Improving the Application of High Molecular Weight Biotinylated Dextran Amine for Thalamocortical Projection Tracing in the Rat

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高分子量生物素化葡聚糖胺在大鼠丘脑-皮质投影示踪中的应用

DOI:

10.3791/55938-v

•

06:39 min

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April 12, 2018

•

Dongsheng Xu*¹, Jingjing Cui*¹, Jia Wang, Zhiyun Zhang, Chen She, Wanzhu Bai

¹Institute of Acupuncture and Moxibustion,China Academy of Chinese Medical Sciences

Chapters

00:04Title
00:42Stereotaxic Injection of Tracer
02:54Perfusion and Sectioning
04:15Double Fluorescent Staining for BDA and Basic Neural Elements in Cerebral Cortex
05:07Results: Injection Site and Typical Neural Labeling with High Molecular Weight BDA in the Somatosensory Cortex
06:04Conclusion

Summary

자동 번역

在这里, 我们提出了一个完善的协议, 以有效地揭示生物素化葡聚糖胺 (BDA) 标签的荧光染色方法通过一个相互的神经通路。它适用于分析 BDA 标签的精细结构, 并在共焦激光扫描显微镜下将其与其他神经元进行区分。

Tags

Biotinylated Dextran Amine Neural Labeling Thalamocortical Projection Rat Neural Circuits Microsyringe Stereotaxic Craniotomy BDA Injection

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