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Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach

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Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach

Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach

DOI:

10.3791/55946-v

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11:12 min

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September 11, 2017

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Jakob Frimodt-Møller, Godefroid Charbon, Karen A. Krogfelt, Anders Løbner-Olesen

¹Department of Biology, Section for Functional Genomics and Center for Bacterial Stress Response and Persistence (BASP),University of Copenhagen, ²Department of Microbiology and Infection Control,Statens Serum Institut

Chapters

00:05Title
00:48Collection of Transposon Library
02:49Competition Experiment in LB
03:50Southern Blot Analysis to Monitor Competition Over Time
04:59Identification of the Fittest Clones
07:06Flow Cytometry
08:53Results: Transposon-mediated Insertion of DNA Element in E. coli
10:41Conclusion

Summary

자동 번역

Here, the power of a transposon-mediated random insertion of a non-coding DNA element was used to resolve its optimal chromosomal position.

Tags

Keywords: Escherichia Coli DNA Element Transposon Chromosomal Location Competition Experiments Whole Genome Sequencing DARS2 Region Electrocompetent Cells Electroporation Kanamycin Transposon Library

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