/

/

用新的转介导法测定大肠杆菌中 DNA 元素的最佳染色体位置 (s)

JoVE 신문
유전학

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

JoVE 신문 유전학

Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach

Please note that all translations are automatically generated. Click here for the English version.

用新的转介导法测定大肠杆菌中 DNA 元素的最佳染色体位置 (s)

DOI:

10.3791/55946-v

•

11:12 min

•

September 11, 2017

•

Jakob Frimodt-Møller, Godefroid Charbon, Karen A. Krogfelt, Anders Løbner-Olesen

¹Department of Biology, Section for Functional Genomics and Center for Bacterial Stress Response and Persistence (BASP),University of Copenhagen, ²Department of Microbiology and Infection Control,Statens Serum Institut

Chapters

00:05Title
00:48Collection of Transposon Library
02:49Competition Experiment in LB
03:50Southern Blot Analysis to Monitor Competition Over Time
04:59Identification of the Fittest Clones
07:06Flow Cytometry
08:53Results: Transposon-mediated Insertion of DNA Element in E. coli
10:41Conclusion

Summary

자동 번역

在这里, 转介导的随机插入编码 DNA 元素的力量被用来解决它的最佳染色体位置。

Tags

Keywords: Escherichia Coli DNA Element Transposon Chromosomal Location Competition Experiments Whole Genome Sequencing DARS2 Region Electrocompetent Cells Electroporation Kanamycin Transposon Library

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

使用了大气颗粒物引起的染色体畸变分析<em>体外</em>系统

单细胞测序拷贝数改变的检测

通过DNA含量测定的哺乳动物复制时序的全基因组的测定

植物标本标本的鲁棒 DNA 分离与高通量测序库建设

一种新的饱和诱变方法: 利用 Phosphorothioates 对 RNA 调控蛋白结合位点的单步表征

可抽税抗生素耐药盒 P1 转导制备大肠杆菌中的基因缺失

利用体外和细胞内形状研究小分子诱导的预 mrna 结构变化

remtoe 控制系统在内源基因表达调控中的应用

基于质谱的蛋白质组学分析, 利用 OpenProt 数据库揭开从非规范开放阅读框架翻译的新蛋白质

在彗星分析中使用冷冻组织评估DNA损伤

Read Article