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Purification of the Membrane Compartment for Endoplasmic Reticulum-associated Degradation of Exogenous Antigens in Cross-presentation
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JoVE 신문 면역학 및 감염병학
Purification of the Membrane Compartment for Endoplasmic Reticulum-associated Degradation of Exogenous Antigens in Cross-presentation

Purification of the Membrane Compartment for Endoplasmic Reticulum-associated Degradation of Exogenous Antigens in Cross-presentation

DOI:
10.3791/55949-v

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12:48 min

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August 21, 2017

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, , ,
1Laboratory of Physiological Chemistry, Faculty of Pharmacy,Takasaki University of Health and Welfare

Chapters

  • 00:05Title
  • 01:13Preparation of Microsomes
  • 04:44Purification of Microsomes with bOVA Underground ERAD
  • 06:59In Vitro Reconstruction of ERAD Ubiquination of bOVA Using Purified Microsomes
  • 09:04Results: Purified Microsomes and In Vitro Ubiquination of the Exogenous Antigen
  • 11:06Conclusion

Summary

자동 번역

자동 번역

The method described here is a new vesicle isolation protocol, which allows for the purification of the cellular compartments where exogenous antigens are processed by endoplasmic reticulum-associated degradation in cross-presentation.

Tags

Keywords: Membrane CompartmentEndoplasmic Reticulum-associated Degradation (ERAD)Cross-presentationExogenous AntigenDendritic CellsBiotinylated Ovalbumin (bOVA)Vesicle IsolationMicrosomeUbiquitination

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