JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
1Department of Molecular Physiology and Biophysics,University of Virginia School of Medicine, 2Robert M. Berne Cardiovascular Research Center,University of Virginia School of Medicine, 3Department of Hematology,St. Jude Children’s Research Hospital, 4Research Histology Core,University of Virginia School of Medicine
Chapters
- 00:05Title
- 00:49Plating Cells for Vascular Cell co-culture
- 04:14Harvesting Fractions from the VCCC
- 07:21Harvesting and Embedding the VCCC for Transverse Immunofluorescence
- 09:11Results: Myoendothelial Junction Formation in Vascular Cell Co-cultures
- 10:18Conclusion
Een cel cultuur model van weerstand slagaders wordt beschreven, waardoor de dissectie van signalering van trajecten in endotheel, gladde spieren, of tussen endotheel en gladde spieren (de kruising van de myoendothelial). De selectieve toepassing van agonisten of eiwit isolatie, elektronenmicroscopie of immunofluorescentie kan worden gebruikt met behulp van deze cel cultuur model.
