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Chapters
- 00:05Title
- 00:35Cell Seeding
- 02:00Cell Transfection and Nocodazole Treatment
- 02:50Image Acquisition of Multi-color Beads and Golgi Mini-stacks
- 04:36Image Analysis, Acquire Centers of Fluorescent Beads
- 07:57Select and Measure Golgi Mini-stacks
- 10:59Results: Imaging the Golgi’s Center of Fluorescence Mass
- 12:31Conclusion
The precise localization of Golgi residents is essential for understanding the cellular functions of the Golgi. However, conventional optical microscopy is unable to resolve the sub-Golgi structure. Here we describe the protocol for a conventional microscopy based super-resolution method to quantitatively determine the sub-Golgi localization of a protein.
