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Tumor Engraftment in einem Xenograft-Maus-Modell der menschlichen Mantel-Zell-Lymphom
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JoVE 신문 암 연구학
Tumor Engraftment in a Xenograft Mouse Model of Human Mantle Cell Lymphoma
DOI:

10:52 min

March 30, 2018

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Chapters

  • 00:05Title
  • 01:23Preparation of Primary Peripheral Blood Mononuclear Cells by Density Gradient Separation
  • 03:15Enrichment of B Cells Negative Selection
  • 05:34Development of the Xenograft Mouse Model
  • 06:07Chimerism Analysis of Tumor Cell Engrafted in Different Organs
  • 07:43Results: Use of Flow Cytometry to Analyze the B Cell Enrichment in Mice Xenografts Obtained from Mantle Cell Lymphoma Patients
  • 09:08Conclusion

Summary

자동 번역

Mantel-Zell-Lymphom (MCL) ist ein schwer zu B-Zell-Störung zu behandeln, und es ist ebenso schwierig, zur Einrichtung eines Xenograft-Maus-Modell der primären MCL zu studieren und Therapeutika zu entwickeln. Hier beschreiben wir die erfolgreiche Etablierung von MCL Xenotransplantate bei Mäusen zu helfen, die zugrunde liegende Biologie zu verstehen.

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