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Chapters
- 00:05Title
- 00:41Day 1: Primary Immunostaining of Mouse Muscle Sections
- 01:57Day 2: Secondary Immunostaining of Mouse Muscle Sections
- 03:24Collecting Muscle Images and their Analysis with the Fiber Quantification Macro
- 06:23Results: Representative Immunostaining and Morphometric Analysis
- 07:50Conclusion
Immunohistochemical staining of myosin heavy chain isoforms has emerged as the state-of-the-art discriminator of skeletal muscle fiber-type (i.e., type I, type IIA, type IIX, type IIB). Here, we present a staining protocol along with a novel semi-automated algorithm that facilitates rapid assessment of fiber-type and fiber morphology.
