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小鼠骨骼肌形态及纤维型成分的半自动化分析
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Semi-automated Analysis of Mouse Skeletal Muscle Morphology and Fiber-type Composition
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小鼠骨骼肌形态及纤维型成分的半自动化分析

DOI:
10.3791/56024-v

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08:36 min

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August 31, 2017

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, , , ,
1Department of Medicine-Cardiology Division,University of Colorado School of Medicine, 2Department of Molecular Physiology and Biophysics,Baylor College of Medicine

Chapters

  • 00:05Title
  • 00:41Day 1: Primary Immunostaining of Mouse Muscle Sections
  • 01:57Day 2: Secondary Immunostaining of Mouse Muscle Sections
  • 03:24Collecting Muscle Images and their Analysis with the Fiber Quantification Macro
  • 06:23Results: Representative Immunostaining and Morphometric Analysis
  • 07:50Conclusion

Summary

자동 번역

자동 번역

肌球蛋白重链异构体的免疫组织化学染色已成为骨骼肌纤维型 (即、i 型、类型 IIA、类型 IIX、类型 IIB) 的最先进的鉴别器。在这里, 我们提出了一个染色协议和一个新的半自动算法, 促进纤维型和纤维形态的快速评估。

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