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Chapters
- 00:05Title
- 00:41Day 1: Primary Immunostaining of Mouse Muscle Sections
- 01:57Day 2: Secondary Immunostaining of Mouse Muscle Sections
- 03:24Collecting Muscle Images and their Analysis with the Fiber Quantification Macro
- 06:23Results: Representative Immunostaining and Morphometric Analysis
- 07:50Conclusion
Marquage immunohistochimique des isoformes de la chaîne lourde de myosine a émergé comme le discriminant de l’état-of-the-art des fibres musculaires squelettiques de type (c'est-à-direde type I, type IIA, tapez IIX, type IIB). Nous présentons ici un protocole de coloration avec un nouvel algorithme semi automatisé qui facilite l’évaluation rapide de la morphologie de type fibre et fibre.
