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DNA Nanotubes as a Versatile Tool to Study Semiflexible Polymers
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DNA Nanotubes as a Versatile Tool to Study Semiflexible Polymers

DNA Nanotubes as a Versatile Tool to Study Semiflexible Polymers

DOI:
10.3791/56056-v

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08:00 min

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October 25, 2017

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, , , , , , , ,
1Fraunhofer Institute for Cell Therapy and Immunology, 2Institute of Experimental Physics I,Universität Leipzig

Chapters

  • 00:05Title
  • 01:09Preparation of Filamentous DNA Helix Tubes of Size n
  • 02:41Shear Rheology
  • 04:11Fluorescence-assisted Analysis of DNA Annealing
  • 06:03Results: DNA n-Helix-tube Shear Rheology
  • 07:14Conclusion

Summary

자동 번역

자동 번역

Semiflexible polymers display unique mechanical properties that are extensively applied by living systems. However, systematic studies on biopolymers are limited since properties such as polymer rigidity are inaccessible. This manuscript describes how this limitation is circumvented by programmable DNA nanotubes, enabling experimental studies on the impact of filament rigidity.

Tags

Keywords: DNA NanotubesSemiflexible PolymersSoft MatterBiopolymersFilamentsNetworksHydrogelsCell MigrationStiffnessRheologyDNA HybridizationDNA StrandsThermocyclerSample PreparationDynamic Shear RheometerSample LoadingEvaporation Prevention

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