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基于蛋白质的继承在酿酒酵母中的高通量筛选
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High-throughput Screening for Protein-based Inheritance in S. cerevisiae
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基于蛋白质的继承在酿酒酵母中的高通量筛选

DOI:
10.3791/56069-v

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08:12 min

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August 08, 2017

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,
1Department of Developmental Biology,Stanford University School of Medicine, 2Department of Chemical and Systems Biology,Stanford University School of Medicine

Chapters

  • 00:05Title
  • 00:33Initial Overexpression
  • 03:12Identifying Cultures Affected by the Stressor
  • 05:13Results: Identification of ORFs that Modulate Protein-based Inheritance
  • 06:45Conclusion

Summary

자동 번역

자동 번역

此协议说明一种高通量方法来功能屏幕在酿酒酵母中的蛋白质基础继承。

Tags

High-throughput ScreeningProtein-based InheritanceS. CerevisiaePrionProtein Over-expressionPhenotypic Memory96-well Plate384-well PlateLiquid Handling RobotOptical DensityManganese ChlorideSD-URASCal-URAST-URA

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