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筛选生物活性纳米颗粒在噬菌体免疫细胞中的Toll样受体信号传导抑制剂
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Screening Bioactive Nanoparticles in Phagocytic Immune Cells for Inhibitors of Toll-like Receptor Signaling
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筛选生物活性纳米颗粒在噬菌体免疫细胞中的Toll样受体信号传导抑制剂

DOI:
10.3791/56075-v

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09:51 min

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July 26, 2017

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, , , ,
1Department of Respiratory Medicine, Shanghai First People’s Hospital,Shanghai Jiaotong University School of Medicine, 2Department of Pediatrics,BC Children’s Hospital and University of British Columbia

Chapters

  • 00:05Title
  • 00:57Preparation of Cell Culture Media and Reagents
  • 02:10Culture of THP-1 Reporter Cell-derived Macrophages
  • 03:45Screening for Potential TLR4 Nano-inhibitors Using the Reprter Cells
  • 07:22Results: Inhibition of Toll-like Receptor Signaling
  • 09:13Conclusion

Summary

자동 번역

자동 번역

Toll样受体（TLR）信号在许多人类炎性疾病的病理生理学中起着重要作用，并且预期在许多炎症状态下调节生物活性纳米颗粒的TLR应答是有益的。基于THP-1细胞的报道细胞提供了一个通用和强大的筛选平台，用于鉴定TLR信号传导的新型抑制剂。

Tags

Bioactive NanoparticlesToll-like Receptor SignalingInnate Immune CellsInflammatory DiseasesPhagocytic Immune CellsReporter Cell SystemsNanoinhibitorsScreening ProcedureLuciferase SubstrateSEAP SubstrateCell CulturePMA

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