The <em>C. elegans</em> Intestine As a Model for Intercellular Lumen Morphogenesis and <em>In Vivo </em>Polarized Membrane Biogenesis at the Single-cell Level: Labeling by Antibody Staining, RNAi Loss-of-function Analysis and Imaging

Nan Zhang; Liakot A Khan; Edward Membreno; Gholamali Jafari; Siyang Yan; Hongjie Zhang; Verena Gobel

doi:10.3791/56100

Journal

/

발생학

/

C. 线虫小肠作为细胞间腔形态发生和体内极化膜生物单细胞水平的模型: 抗体染色、rna 干扰功能分析和成像标记

JoVE 신문
발생학

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

JoVE 신문 발생학

The C. elegans Intestine As a Model for Intercellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis at the Single-cell Level: Labeling by Antibody Staining, RNAi Loss-of-function Analysis and Imaging

Please note that all translations are automatically generated. Click here for the English version.

C. 线虫小肠作为细胞间腔形态发生和体内极化膜生物单细胞水平的模型: 抗体染色、rna 干扰功能分析和成像标记

DOI:

10.3791/56100-v

•

12:15 min

•

October 03, 2017

•

Nan Zhang², Liakot A Khan, Edward Membreno, Gholamali Jafari, Siyang Yan, Hongjie Zhang³, Verena Gobel

¹Mucosal Immunology and Biology Research Center, Developmental Biology and Genetics Core, Massachusetts General Hospital,Harvard Medical School, ²College of Life Sciences,Jilin University, ³Faculty of Health Sciences,University of Macau

Chapters

00:05Title
01:24Introduction to the Accompanying Publication
02:47Nematode Fixation
04:19Nematode Antibody Staining
06:33Applying RNAi to Nematodes Using a Feeding Protocol
07:57Analyzing Phenotypes Using Fluorescence Dissecting Microscopy
08:41Analyzing Phenotypes Using Confocal Microscopy
10:14Results: Immunohistochemistry, RNAi and Microscopic Analysis of Intestinal Apical membrane and Lumen Morphogenesis
11:45Conclusion

Summary

자동 번역

透明的C. 线虫小肠可作为 "体内组织室", 用于研究多细胞 tubulogenesis 中的单细胞和亚 apicobasal 膜和腔生物。本协议描述了如何结合标准标记, 功能的遗传/rna 干扰和微观的方法来解剖这些过程的分子水平。

C. 线虫小肠作为细胞间腔形态发生和体内极化膜生物单细胞水平的模型: 抗体染色、rna 干扰功能分析和成像标记

•

•

•

Chapters

Summary

자동 번역

Tags

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

在GFP-转基因斑马鱼使用Photoconvertible PSmOrange系统追踪单元

成像清除胚胎和出生后心脏在单细胞分辨率

方法来检查淋巴腺和血细胞中<em>果蝇</em>幼虫

在Active ERK的时空分析<em>℃。线虫</em>生殖系

C. 线虫排泄管作为细胞内腔形态发生和体内极化膜生物在单个细胞中的模型: GFP-融合、rna 干扰界面和成像的标记

成人果蝇脑内蘑菇体和感光神经元的解剖和荧光染色

rna 干扰和热休克诱导转录因子表达在重编程生殖细胞到神经元中的应用C. 线虫

CRISPR 介导的小脑颗粒细胞在宫内电穿孔基因转移中的功能分析损失

评估溶酶体碱化在活的秀丽线虫中的肠道

种系线虫与胸苷模拟教育的细胞周期分析

Read Article