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Biocontainmentにおけるウイルスサンプルの陰性染色のためのカプセルの利用
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Utilization of Capsules for Negative Staining of Viral Samples within Biocontainment
DOI:

08:22 min

July 19, 2017

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Chapters

  • 00:05Title
  • 01:08Experiment Preparation in a BSL-2 Environment Prior to Working with the Virus Samples (Biocontainment Procedures were Filmed in BSL-4 Training Lab)
  • 01:45The Capsule Method for Negative Staining in Biocontainment Using Aqueous Glutaraldehyde and 1% Osmium Tetroxide Vapor Inactivation
  • 04:24The Capsule Method for Inactivation in Biocontainment 2% Glutaraldehyde Followed by Negative Staining in BSL-2 Laboratory
  • 05:50Results: Analyzing the Capsule Method of Negative Staining Viral Samples
  • 07:14Conclusion

Summary

자동 번역

このプロトコルは、BSL-2、-3、または-4の実験室で容易に使用することができる陰性染色ウイルスサンプルのための指示を提供する。これには透過電子顕微鏡グリッドを保護する革新的な処理カプセルの使用が含まれ、生化学の中でより乱暴な環境での取り扱いが容易になります。

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