Isolering og karakterisering af primære rotte ventil interstitielle celler: en ny Model til at studere aortaklappen forkalkning

Article DOI: 10.3791/56126-v • 08:55 min • November 20th, 2017

November 20th, 2017 •

Cui Lin¹, Dongxing Zhu², Greg Markby¹, Brendan M. Corcoran³, Colin Farquharson¹, Vicky E. Macrae¹

¹Developmental Biology, The Roslin Institute and R(D)SVS, University of Edinburgh, ²Guangzhou Institute of Cardiovascular Disease, The Second Affiliated Hospital, School of Basic Medical Sciences, Guangzhou Medical University, ³Clinical Sciences and R(D)SVS, University of Edinburgh

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Denne protokol beskriver isolation, kultur og forkalkning af rotte-afledte ventil interstitielle celler, en yderst fysiologiske i vitro model af forkalkede aortaklappen sygdom (CAVD). Udnyttelse af denne rotte model letter CAVD forskning i udforskning af celle og molekylære mekanismer, der ligger til grund for denne komplekse patologiske proces.

Isolering og karakterisering af primære rotte ventil interstitielle celler: en ny Model til at studere aortaklappen forkalkning

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