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立体定向激光捕获创伤性脑区显微解剖

JoVE 신문
신경과학

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JoVE 신문 신경과학

Stereotactic Atlas-Guided Laser Capture Microdissection of Brain Regions Affected by Traumatic Injury

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立体定向激光捕获创伤性脑区显微解剖

DOI:

10.3791/56134-v

•

09:29 min

•

September 11, 2017

•

Harris A. Weisz*¹, Deborah R. Boone*¹, Stacy L. Sell, Helen L. Hellmich

¹Department of Anesthesiology,University of Texas Medical Branch

Chapters

00:05Title
00:59Region Identification and Sectioning
04:01Staining Protocol
04:50Stereotactic Atlas Guided Laser Capture Microdissection with the LCM System
07:36Results: LCM of Traumatic Brain Injury affected Brain Regions
08:55Conclusion

Summary

자동 번역

我们描述了利用激光捕获显微切割, 获得不同的细胞群体的样本, 从各个脑区的基因和 rna 分析。这项技术可以研究大鼠脑部特定区域创伤性脑损伤的差异效应。

Tags

Stereotactic Atlas Laser Capture Microdissection Traumatic Brain Injury Frontal Cortex Hippocampus Molecular Analysis Transcriptional Analysis Cryostat Optimal Cutting Temperature Medium Brain Regions Cell Types

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