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Evaluation of Synapse Density in Hippocampal Rodent Brain Slices

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Evaluation of Synapse Density in Hippocampal Rodent Brain Slices

Evaluation of Synapse Density in Hippocampal Rodent Brain Slices

DOI:

10.3791/56153-v

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07:44 min

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October 06, 2017

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Faye McLeod*¹, Aude Marzo*¹, Marina Podpolny, Soledad Galli, Patricia Salinas

¹Department of Cell and Developmental Biology,University College London

Chapters

00:05Title
00:41Hippocampal Slice Preparation
02:17Immunofluorescence for Synaptic Markers
04:34Imaging Using Confocal Laser Scanning Microscopy
06:04Synapse Density in Hippocampal Slices from iDkk1 Mice
07:17Conclusion

Summary

자동 번역

A protocol for accurately identifying and analyzing synapses in hippocampal slices using immunofluorescence is outlined in this article.

Tags

Immunostaining Synapse Density Hippocampus Rodent Brain Slices Neurodegenerative Diseases Vibratome ACSF Paraformaldehyde Immunofluorescent Staining VGlut1 Antibody Secondary Antibodies

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