Journal
/
생물학
/
Optical Cross-Sectional Muscle Area Determination of Drosophila Melanogaster Adult Indirect Flight Muscles
JoVE 신문
생물학
Author Produced
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생물학
Optical Cross-Sectional Muscle Area Determination of Drosophila Melanogaster Adult Indirect Flight Muscles

Optical Cross-Sectional Muscle Area Determination of Drosophila Melanogaster Adult Indirect Flight Muscles

DOI:
10.3791/56179-v

•

06:43 min

•

March 31, 2018

•

, ,
1Department of Genetics and Interdisciplinary Research Structure for Biotechnology and Biomedicine (BIOTECMED),University of Valencia, 2Incliva Health Research Institute, 3Joint unit CIPF-Incliva

Chapters

  • 00:00Title
  • 00:32Fixation and resin embedding
  • 02:53Trimming and sectioning of blocks
  • 03:45Staining IFM sections
  • 04:06Mounting IFM sections
  • 04:26Acquisition of images and quantification of muscle area
  • 05:10Representative results
  • 06:05Conclusions

Summary

자동 번역

자동 번역

We report a method to quantify muscle area, which is an indirect method to determine muscle mass in Drosophila adults. We demonstrate the application of our methodology by analyzing the indirect flight muscles in a Drosophila model of Myotonic Dystrophy disease.

Tags

Keywords: Drosophila MelanogasterOptical Cross-sectional Muscle AreaIndirect Flight MusclesMuscle MassMuscle AtrophyDorsal Longitudinal MusclesFixationResin EmbeddingAnesthesiaDehydrationPropylene OxideResin PolymerizationTrimmingSectioningUltramicrotome

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/56179?article_type=v

Dissection of Larval CNS in Drosophila Melanogaster
check_url/kr/56179?article_type=v

High-Resolution Video Tracking of Locomotion in Adult Drosophila Melanogaster
check_url/kr/56179?article_type=v

Visually Mediated Odor Tracking During Flight in Drosophila
check_url/kr/56179?article_type=v

Dissection of Oenocytes from Adult Drosophila melanogaster
check_url/kr/56179?article_type=v

Paraffin-Embedded and Frozen Sections of Drosophila Adult Muscles
check_url/kr/56179?article_type=v

Isolation of Drosophila melanogaster Testes
check_url/kr/56179?article_type=v

Insulin Injection and Hemolymph Extraction to Measure Insulin Sensitivity in Adult Drosophila melanogaster
check_url/kr/56179?article_type=v

Ex Vivo Assessment of Contractility, Fatigability and Alternans in Isolated Skeletal Muscles
check_url/kr/56179?article_type=v

Measurement of Lifespan in Drosophila melanogaster
check_url/kr/56179?article_type=v

Maintenance of a Drosophila melanogaster Population Cage

Read Article