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JoVE Journal Genetics
CRISPR/Cas9 蛋白注射到沟鲶、鮰松毛虫、基因编辑的胚胎
 
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CRISPR/Cas9 蛋白注射到沟鲶、鮰松毛虫、基因编辑的胚胎

Article DOI: 10.3791/56275-v • 16:08 min • January 20th, 2018
January 20th, 2018 •
*1,2, *1,3, 1, 4, 4, 5, 5, 1
1School of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, 2Department of Animal Wealth Development, Faculty of Veterinary Medicine, Suez Canal University, 3Anatomy and Embryology Department, Faculty of Veterinary Medicine, Cairo University, 4Department of Molecular Physiology and Biophysics, Vanderbilt University, 5Life Science Institute, University of Michigan
* These authors contributed equally

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本文介绍了一种简单高效的 CRISPR/Cas9 系统在沟鲶胚胎基因编辑中的微注射协议。在这个协议中, 引导 rna 和 Cas9 蛋白被微量到一细胞胚胎的卵黄中。通过敲出两条沟鲶免疫相关基因来验证这个协议。

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遗传学 问题 131 CRISPR/Cas9 基因编辑 基因敲除 微注射 沟鲶胚胎 突变 被截断的蛋白质 indels
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