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1Biochemistry and Molecular Biology Department, Faculty of Veterinary medicine,Complutense University, 2University Institute for Neurochemistry Research (IUIN), 3Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC), 4Institute of Stem Cell Research, Helmholtz Center Munich, Neuherberg/Munich, Germany Physiological Genomics, Biomedical Center,Ludwig-Maximilians University Munich, 5Toxicology and Pharmacology Department, Faculty of Veterinary medicine,Complutense University, 6Brain Institute,Federal University of Rio Grande do Norte, 7Department of Biosystems Science and Engineering,Eidgenössische Technische Hochschule (ETH) Zurich
Chapters
- 00:05Title
- 01:14Live Imaging by Time-lapse Video-microscopy
- 04:02Post-imaging Immunocytochemistry, Data Collection, and Processing
- 06:38Single-cell Tracking
- 08:56Results: Example of aNSCs Isolated from the SEZ and Analyzed by Live Imaging and Single Cell Tracking
- 10:17Conclusion
È descritto un protocollo robusto per monitorare le popolazioni neurali di time-lapse video-microscopia seguita da basati su software di post-elaborazione. Questo metodo rappresenta un potente strumento per identificare gli eventi biologici in una popolazione selezionata durante esperimenti dal vivo di formazione immagine.
